M2 Biotherapies : Tissue, Cell, Gene

Boniotto Michele , Bruno Colombo, Christine Baldeschi; Natacha Bessis; Mario Ollero.

The unit comprehends an individual work part and a presential work part. Individual work includes the setup of personal interviews with the future supervisor. During these interviews, the project must be discussed, and a ten-minute presentation must be prepared. Presential work corresponds to attendance to several presentation sessions, usually over two days, depending on the number of students in the Research sub-specialty. All students in the specialty attend the sessions and listen to all presentations and discussions. Each student presents his/her internship project in ten minutes. This is followed by a ten-minute discussion with a jury constituted by several members of the Master’s faculty. The jury establishes an average grade considering presentation skills and discussion.

The aim of this unit is for students to become familiar with their future research work within the laboratory training internship. This includes several key points: first, a dialogue must be established between the student and his/her internship supervisor; second, a tentative project must be proposed, including clear objectives, methodology, experimental plan, potential pitfalls and changes in the original objectives; third, a critical view on the project by the student is encouraged; four, as the project must be presented in public this will build the communication skills of the student; five, this is the right time to challenge the project itself, to unveil the weak points, to anticipate potential difficulties that the student can encounter throughout the internship. This is a first chance to evaluate the scientific skills of students, and therefore a reference point to eventually evaluate the progress attained during the internship. The presence of students at presentations and discussions helps develop a critical attitude on research projects, and gives them the chance to broaden their knowledge on state-of-the-art research on biotherapies.

To follow this unit students must have followed the rest of teaching units corresponding to the Research sub-specialty. Previously, they must have presented an article related to their internship project within UE8. They must have found a host laboratory for their research internship.

Novembre - Décembre.

UPEC

Boniotto Michele, Bruno Colombo; Christine Baldeschi; Mario Ollero; Natacha Bessis.

Individual work includes a series of personal interviews with the future supervisor. The supervisor will propose a relevant bibliography to the internship project and one research article will be chosen for presentation. The student will prepare a ten-minute presentation to describe synthetically the selected article, including a critical view of the work. Presential work corresponds to attendance to several presentation sessions, usually over three days. All students in each sub-specialty attend the sessions and listen to all presentations and discussions. Each student presents his/her chosen article project in ten minutes. This is followed by a ten-minute discussion with a jury constituted by several members of the Master’s faculty. The jury establishes an average grade considering presentation skills and discussion. A second written exercise takes place during exam sessions, in which an incomplete article is provided to students. In four hours, they must perform a written analysis of the article following a series of key questions.

The primary aim of the unit is to develop oral communication skills. Additional aims for students are to become familiar both with their future research work within the laboratory training internship and with the current literature in the field. This includes several key points: first, a dialogue must be established between the student and his/her internship supervisor; second, the most relevant articles to the future internship topic and subsequent extension of knowledge in one specific field of biotherapies; third, students will train in oral presentation by constructing a visual support, developing the ability to synthesize and communicate a scientific work; fourth, a critical view on the presented work by the student is encouraged. This is a first chance to evaluate the communication skills of students, and therefore a reference point to eventually evaluate the progress attained during the internship. The presence of students at presentations and discussions helps develop a critical attitude on published scientific work, and gives them the chance to broaden their knowledge on state-of-the-art research on biotherapies.

To follow this unit students must have followed the rest of teaching units corresponding to the Research sub-specialty. They must have found a host laboratory for their research internship, since the presented work must be linked with the future internship project, and discussed with the internship supervisor.

Décembre.

UPEC

Philippe Le Corvoisier; Violaine Closson; Isabelle Sainte Marie; Claire Pressiat; Christian PINSET; Catarina Madeira; Katia Ledudal; Romain Gallet; Anne Marie Taburet; Alexandra Meyer.

Cet UE contient 17 cours soit 30h d'enseignement.

Le but de ce cours est de présenter aux étudiants les questions méthodologiques et juridiques spécifiques à la recherche clinique dans le domaine des biothérapies.

Le CV des étudiants est évalué par un comité pédagogique.

Décembre.

UPEC

Florence Gonnet; Francis Quetier; Marie Anne Debily; Mario Amendola; Valérie Chaudru.

The UE includes 7 class (10,5h).

The aim of this class is to introduce proteomic, transcriptom, sequencing and gene editing.

None.

Recent publications in the field.

Décembre.

EVRY

Individual work includes a series of personal interviews with the future supervisor. The supervisor will propose a relevant bibliography to the internship project and one research article will be chosen for presentation. The student will prepare a ten-minute presentation to describe synthetically the selected article, including a critical view of the work. Presential work corresponds to attendance to several presentation sessions, usually over three days. All students in each sub-specialty attend the sessions and listen to all presentations and discussions. Each student presents his/her chosen article project in ten minutes. This is followed by a ten-minute discussion with a jury constituted by several members of the Master’s faculty. The jury establishes an average grade considering presentation skills and discussion. A second written exercise takes place during exam sessions, in which an incomplete article is provided to students. In four hours, they must perform a written analysis of the article following a series of key questions.

The primary aim of the unit is to develop oral communication skills. Additional aims for students are to become familiar both with their future research work within the laboratory training internship and with the current literature in the field. This includes several key points: first, a dialogue must be established between the student and his/her internship supervisor; second, the most relevant articles to the future internship topic and subsequent extension of knowledge in one specific field of biotherapies; third, students will train in oral presentation by constructing a visual support, developing the ability to synthesize and communicate a scientific work; fourth, a critical view on the presented work by the student is encouraged. This is a first chance to evaluate the communication skills of students, and therefore a reference point to eventually evaluate the progress attained during the internship. The presence of students at presentations and discussions helps develop a critical attitude on published scientific work, and gives them the chance to broaden their knowledge on state-of-the-art research on biotherapies.

To follow this unit students must have followed the rest of teaching units corresponding to the Research sub-specialty. They must have found a host laboratory for their research internship, since the presented work must be linked with the future internship project, and discussed with the internship supervisor.

Novembre.

UPEC

Stéphane Blot, Lydia Danglot, Marc Denhain, Thomas Hubert, Pilippe Lecorvoisier, Andrew Tindall.

The UE includes 7 class.

At the end of these lectures, students should be able to choose the most appropriated animal model to design biotherapeutical protocols. This ECUE show a panoply of animal models the most often used underlining the pros and cons of each one.

Topics:
Animal models of heart failure
Animal models in psychiatry
Aquatic models for studying human diseases and to screen human therapeutics and environmental toxicants.
Mutants animals: identification, breeding and analysis
Neurodegenerating diseases
Preclinical large models of degenerative myopathies, example of Duchenne muscular dystrophy
Translational research on diabetes: endocrine and metabolic surgery, from pig to patient.

Basic fondamentals of physiology, genetics, immunology and cellular and molecular biology.

Recent publications in the field.

Novembre - Décembre.

UPEC

Laetitia Aubry; Christine Baldeschi; BARRITAULT Denis; Benihoud Karim; Bigey Pascal; François Jean Christophe; Goyenvalle Aurélie ; Holic-Barlet Nathalie; Marie Corinne; Monville Christelle; Nait-Oumesmar Brahim; Ronzitti Giuseppe; Tournois Johana.

The UE includes 20 class.

The aim of this class is to introduce fundamental basic of biothérapies with a focus on vector choice for gene therapy and cell therapy

Topics:
Vector choice for gene therapy
AAV vectors
Retroviral vectors
Adenoviral vectors
Non-viral vectors and clinical trials
Antisense ribozymes RNAi
RNA splicing modulation

Stem Cells for cell therapy
Pluripotent stem Cells and the eye
Pluripotent Stem Cells and skin
Muscle stem cells.

Basic fondamentals of cell biology.

Recent publications in the field.

Novembre.

EVRY

Christina Zeitz; Thierry Leveillard; Violaine Plante; Nadège Bondurand; Sabrina Belbekhouche; Martina Merinello; Fabrice Lejeune; Matthias Titeux; Valerie Urbach.

The unit is composed of nine lectures of one and a half hours each. Four lectures per day are scheduled. All of them take place at the School of Medicine in Créteil (UPEC). Students are provided beforehand with handouts of presentations. Lectures have the structure of a scientific seminar, in which the speaker gives an introduction on the problem, then presents the state of the art of research and technologies in the field, and shows the main results of his/her own research. The speakers recommend bibliography for further reading. The exam takes place along with that of the other units. The students can provide the coordinator with feedback about the lectures.

The aim of this unit is to provide the students with the latest developments in basic and preclinical research in therapies for specific genetic diseases. The unit is divided into different modules, each corresponding to a disease caused by gene mutations that result in dysfunction of the retina, brain and nervous system (neuropathy and neurocristopathy), neuromuscular diseases and cystic fibrosis. Two independent modules deal with correction of nonsense mutation as an approach to genetic diseases and a targeted drug delivery approach. In all cases, an introduction is given to the fundamentals of the diseases, then a recent historical evolution of the main therapeutic approaches, and the latest developments. This includes a critical view of the state of the art, with the pros and cos of what is currently available, and a prospective view on the next advancements. Lectures are given by invited specialists. The students are confronted to the state-of-the-art research, in some cases having access to firsthand information even before publication. The interaction between students and specialists is encouraged.

To follow this unit students must have acquired the principles of genetics and molecular biology techniques. A strong background in cell biology, molecular biology and physiology is a must. Proficiency in immunology fundamentals is extremely helpful.

Recent publications in the field.

Novembre.

UPEC

Alain Chapel; Philippe Le Corvoisier; Karim SI Tayeb; Hélène Rouard; Thomas Hubert; Amelie Wegener; Daigo Sawaki; Olivier Detente; Laetitia Aubry; Andreas Schedl;.

The unit is composed of twelve lectures of one and a half hours each. Four lectures per day are scheduled. All of them take place at the School of Medicine in Créteil (UPEC). Students are provided beforehand with handouts of presentations. Lectures have the structure of a scientific seminar, in which the speaker gives an introduction on the problem, then presents the state of the art of research and technologies in the field, and shows the main results of his/her own research. The speakers recommend bibliography for further reading. The exam takes place along with that of the other units. The students can provide the coordinator with feedback about the lectures.

The aim of this unit is to provide the students with the latest developments in basic and preclinical research in substitutive therapies, cell therapies and tissue repair therapies. The unit is divided into different modules, each corresponding to a tissue or systems: skeletal and cardiac muscle myopathies, lug diseases, neurodegenerative diseases, liver diseases, renal diseases, cartilage and bone diseases, pancreatic disease, stroke, as well as a module devoted to accidental injuries, such as exposure to radiation and to burns. In all cases, an introduction is given to the fundamentals of cell and tissue repair in the corresponding system, then a recent historical evolution of the main therapeutic approaches, and the latest developments. This includes a critical view of the state of the art, with the pros and cos of what is currently available, and a prospective view on the next advancements. Lectures are given by invited specialists. The students are confronted to the state-of-the-art research, in some cases having access to firsthand information even before publication. The interaction between students and specialists is encouraged.

To follow this unit students must have acquired the principles of organ development and stem cell biology. A strong background in cell biology, molecular biology and genetics is a must. Proficiency in immunology fundamentals is extremely helpful.

Recent publications in the field.

Novembre.

UPEC

H Rouard, K Sii Felice, S Maury, J Cartron, S Chtourou, L Douay, D Baruch, Z Ivanovic,.

The UE is composed of 8 lectures.

This UE is organized by Etablissement Français du Sang (EFS). EFS’s primary mission is to meet the quantity and quality required by a million patients. Over 3.2 million donations must be collected each year in safe conditions for donors and receivers. Blood products have a limited life span. We observed an increased in blood product needs. In contrast, the number of new donors decreases and there are limitations of ressources. Therefore the challenge for EFS is to explore alternative strategies. EFS carries out research activities in cutting-edge fields, contributing to scientific and medical advances in fields related to transfusion activities: research into blood cells, cell and tissue therapy. We proposed in this UE to have scientific reports about blood and cell production in vitro. Thematic included are: in vitro expansion of HSC, Red blood cells and platelets production in vitro, haemoglobin substitutes, CAR T cells, proteins purified from plasma.

None.

Recent publications in the field.

Novembre.

Ilaria Cascone, Bruno Colombo, Sylvain Fisson, Didier Jean, Mathieu Leclerc, Jacques Medioni, Katrina Podsypanina, David Sibon, Mingxing Wei.

Participating teachers: -Ilaria Cascone, Bruno Colombo, Sylvain Fisson, Didier Jean, Mathieu Leclerc, Jacques Medioni, Katrina Podsypanina, David Sibon, Mingxing Wei

Modality of the control of the knowledge (Modalité du contrôle des connaissances, MCC) : -1st session exam: in January, written exam -2nd session exam: in Mach-April, written exam.

This ECUE is dedicated to the mechanisms of carcinogenesis and biotherapeutic treatment strategies. At the end of these lectures, students are able to evaluate the most efficient strategy to fight cancers, based on the nature of cancer cells.

Topics:
Anti-CD20 monoclonal antibody: mechanism of action and clinical applications
Anticancer immunotherapies: dendritic cells-based vaccines
Anticancer therapies targeted against the driver oncogenes
Antileukemic allogeneic T cell therapy
CAR-T: Next-generation cancer immunotherapy
Mesothelioma : Pathogenesis and Biotherapy
Targeting angiogenesis in cancers
Targeting of surface proteins to arrest tumoral growth: development of nanoparticles
Tumor Immunosurveillance in immune sanctuaries.

Basic fondamentals of immunology, genetics and cellular and molecular biology.

Recent publications in the field.

Novembre.

EVRY

Pr P. Decker (Univ Paris 13); Dr J. Biton (Univ Paris 13); Dr B Salomon (INSERM); Pr A Créange (UPEC); Dr JL Teillaud (INSERM); Pr B Colombo (UEVE).

Course in the form of research seminar lasting 1h30 by researchers; in English.

Understand different treatment approaches by immunotherapy in various inflammatory or immune system-related diseases.

Basic fondamentals of immunology.

Recent publications in the field.

Novembre - Décembre.

UPEC

Sessions of at least 2 hours, in some cases full days, in French, with speakers from the private professional field.

To understand the issues in biotherapy industries, the regulatory and financial environment. To study the industrial process of drug discovery, production and marketing. To Know the mechanisms for financing innovation. To know the mechanisms of industrial property protection.
To know the environment of industrial and service companies in the field of biotechnology. To undersdand how research projects work in companies. To know how to evaluate yourself, assess your skills and introduce yourself.

None.

None.

Décembre.

UFR SMBH, Univ Paris 13, Bobigny

André Dan (Challengy); Mohamed Aboudrard (SAIC Univ Paris 13); Alexandre Lebkiri (Cabinet Lebkiri et Camus); Delphine Machy (SAIC Univ Paris 13); Raphael Daccosta.

Sessions of at least 2 hours, in some cases full days, in French, with speakers from the private professional field.

To understand the issues in biotherapy industries, the regulatory and financial environment. To study the industrial process of drug discovery, production and marketing. To Know the mechanisms for financing innovation. To know the mechanisms of industrial property protection.
To know the environment of industrial and service companies in the field of biotechnology. To undersdand how research projects work in companies. To know how to evaluate yourself, assess your skills and introduce yourself.

None.

Décembre.

: UFR SMBH, Univ Paris 13, Bobigny

Philippe Le Corvoisier; Violaine Closson; Isabelle Sainte Marie; Claire Pressiat; Christian PINSET; Catarina Madeira; Katia Ledudal; Romain Gallet; Anne Marie Taburet; Alexandra Meyer.

Cet UE contient 17 cours soit 30h d'enseignement.

Le but de ce cours est de présenter aux étudiants les questions méthodologiques et juridiques spécifiques à la recherche clinique dans le domaine des biothérapies.

Le CV des étudiants est évalué par un comité pédagogique.

Décembre.

UPEC

Janvier - Février - Mars - Avril - Mai - Juin - Juillet.